> The data I have for the KGY37 trim2 screen is
>
> 1b8, 1b11, 1c4, 1c8, 2d10, 2E10, 3D12, 4A3... Not sure if any are the
> flask positives.  Turns out only 1 gave sequence information which  was
> 3D12,  to be Dynactin 1.  For this screen most of the positives could
> not be reconstructed.  No plasmid DNA. for some reason.
>
> The Y190 Trim2 screen.  We screened 5.4 million clones.
>
> Ended up with 35 initial positives from plates.
> only 14 reconfirmed with lacZ on reconstruction
> 7 clones were EFS
> 1 clone  Trim3
> 1 clone Cofilin
> 1 clone RanBPM
> 1 clone e plasminogen activator
> 1 clone Chromosome contig 12
> 1 clone Ubac1
> 1 clone no sequence info
>
> 10 of the 10 flasks had growth.
> 6 of the 10 recomfirmed with lacZ on reconstruction.
> 5 of them where the same clone.  EFS
> 1 was Trim3.
>
>
>
> From this it looks like Y190 is better... Yikes..